Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETS1

Cell type

Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
U-87MG
genotype
control
cell line
U-87MG
chip antibody
ETS1
treatment
TWEAK

Sequenced DNA Library

library_name
GSM6329566
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Diagenode Bioruptor. DNA was sheared to 200-500bp. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer's protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
39565718
Reads aligned (%)
90.4
Duplicates removed (%)
29.9
Number of peaks
1071 (qval < 1E-05)

hg19

Number of total reads
39565718
Reads aligned (%)
89.3
Duplicates removed (%)
30.3
Number of peaks
575 (qval < 1E-05)

Base call quality data from DBCLS SRA