Antigen

Antigen Class

TFs and others

Antigen

ETS1

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U-87MG

genotype

control

cell line

U-87MG

chip antibody

ETS1

treatment

TWEAK

Sequenced DNA Library

library_name

GSM6329566

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Diagenode Bioruptor. DNA was sheared to 200-500bp. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

39565718

Reads aligned (%)

90.4

Duplicates removed (%)

29.9

Number of peaks

1071 (qval < 1E-05)

hg19

Number of total reads

39565718

Reads aligned (%)

89.3

Duplicates removed (%)

30.3

Number of peaks

575 (qval < 1E-05)